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A quantitative western blot method utilizing TMB

A quantitative western blot technique using TMB: Comparability with the usual technique

Fairly a number of molecular natural experiments carried out all by way of the world require the detection or quantification of a protein of curiosity. Western blotting is among the many hottest methods used for this purpose and offers quantitative knowledge with the assistance of specialized software program program. However, its dependence on the picture that is captured, and the background and the absence of a typical protocol forestall the tactic from being completely quantitative. To beat these obstacles, we present a straightforward and reliable assay that is very similar to the frequent technique, excluding the ultimate stage of band visualization and quantification.

We recommend that small objects of the blot that embody the protein of curiosity could be decrease and dipped in a small amount of three,3′,5,5′-Tetramethylbenzidine (TMB) decision, giving a colorimetric signal with linear dependence on the quantity of the protein. The response is stopped with H2 SO4 , and the signal is measured in a plate reader. This modification displays extreme linearity with out additional costs and could be utilized for every purified proteins and proteins current in a lysate. The outcomes obtained with our proposed technique have been in distinction with these obtained by the usual approach and proved to be further reliable. This textual content is protected by copyright. All rights reserved.

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Anti-Galectin-3 Monoclonal Antibody

M00621-1 100ul
EUR 397
Description: Mouse Monoclonal Galectin-3 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

anti-Galectin 1

YF-PA12945 100 ug
EUR 403
Description: Rabbit polyclonal to Galectin 1

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Anti-Galectin 1 Antibody

A00470-2 100ug/vial
EUR 294

anti- Galectin-1 antibody

FNab03314 100µg
EUR 505.25
  • Recommended dilution: WB: 1:500-1:1000
  • IHC: 1:20-1:200
  • Immunogen: lectin, galactoside-binding, soluble, 1
  • Uniprot ID: P09382
  • Gene ID: 3956
  • Research Area: Cell Division and Proliferation, Cardiovascular, Signal Transduction
Description: Antibody raised against Galectin-1

anti- Galectin-1 antibody

FNab03315 100µg
EUR 548.75
  • Recommended dilution: WB: 1:500-1:2000
  • IHC: 1:20-1:200
  • Immunogen: lectin, galactoside-binding, soluble, 1
  • Uniprot ID: P09382
  • Gene ID: 3956
  • Research Area: Cell Division and Proliferation, Cardiovascular, Signal Transduction
Description: Antibody raised against Galectin-1

Anti-Galectin-1 antibody

PAab03314 100 ug
EUR 355

Anti-Galectin-1 antibody

STJ93201 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-1.

Anti-Galectin-1 antibody

STJ98716 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-1.

Anti-Galectin 1 (1A8)

YF-MA13984 100 ug
EUR 363
Description: Mouse monoclonal to Galectin 1

Anti-Galectin 1/Lgals1 Antibody

A00470 100ug/vial
EUR 334

Anti-Galectin 1 Biotinylated Antibody

A00470-Biotin 50ug/vial
EUR 294

Anti-Galectin 1/LGALS1 Antibody

PA1422 100ug/vial
EUR 334

anti-Galectin 1 (1E8-1B2)

LF-MA10174 100 ug
EUR 363
Description: Mouse monoclonal to Galectin 1

Anti-Galectin 1/LGALS1 Antibody

PB9240 100ug/vial
EUR 334

Anti-galectin-1 (mouse) antibody

STJ72519 100 µg
EUR 359

Anti-Galectin-13 (GAL13) / Placental Protein 13 (PP13) Monoclonal Antibody

M08143-1 100ug/vial
EUR 397
Description: Mouse Monoclonal Galectin-13 (GAL13) / Placental Protein 13 (PP13) Antibody. Validated in IHC and tested in Human.

anti-Galectin 3

YF-PA12946 50 ug
EUR 363
Description: Mouse polyclonal to Galectin 3

anti-Galectin 3

YF-PA12947 100 ul
EUR 403
Description: Rabbit polyclonal to Galectin 3

anti-Galectin 3

YF-PA12948 100 ug
EUR 403
Description: Rabbit polyclonal to Galectin 3

anti-Galectin 8

YF-PA12951 50 ul
EUR 363
Description: Mouse polyclonal to Galectin 8

anti-Galectin 13

YF-PA18585 50 ug
EUR 363
Description: Mouse polyclonal to Galectin 13

anti-Galectin 3

YF-PA24079 50 ul
EUR 334
Description: Mouse polyclonal to Galectin 3

anti-galectin 9

YF-PA24081 50 ul
EUR 334
Description: Mouse polyclonal to galectin 9

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

LGALS7 Human, Galectin-7 Human Recombinant Protein, His Tag

PROTP47929-1 Regular: 20ug
EUR 317
Description: Galectin-7 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 156 amino acids (1-136 a.a.) and having a molecular mass of 17.2kDa. ;The Galectin-7 is purified by proprietary chromatographic techniques.

LGALS8 Human, Galectin-8 Human Recombinant Protein, His Tag

PROTO00214-1 Regular: 10ug
EUR 317
Description: LGALS8 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 337 amino acids (1-317 a.a.) and having a molecular mass of 37.9 kDa. The LGALS8 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

LGALS3 Human, Galectin-3 Human Recombinant Protein, His Tag

PROTP17931-1 Regular: 25ug
EUR 317
Description: LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 270 amino acids (1-250 a.a.) and having a molecular mass of 28.3 kDa._x000D_ The LGALS3 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. _x000D_

Anti-human Galectin-3 antibody

STJ15100163 250 µg
EUR 336
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation

Human Galectin-3 (Gal-3) AssayMax ELISA Kit

EG3311-1 96 Well Plate
EUR 477

Human Galectin-4 (Gal-4) AssayMax ELISA Kit

EG3312-1 96 Well Plate
EUR 477

Recombinant Human Galectin-1

7-00427 10µg Ask for price

Recombinant Human Galectin-1

7-00428 50µg Ask for price

Recombinant Human Galectin-1

7-00429 1mg Ask for price

Galectin-1, human recombinant

4648-10
EUR 142

Galectin-1, human recombinant

4648-1000
EUR 1648

Galectin-1, human recombinant

4648-50
EUR 262

Human Galectin-1 (LGALS1)

1-CSB-EP012882HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 30.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Human Galectin-1 (LGALS1)

1-CSB-EP012882HUb1
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 19.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Human Galectin 1 Protein

20-abx066725
  • EUR 620.00
  • EUR 272.00
  • EUR 1859.00
  • EUR 732.00
  • EUR 453.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Anti-Galectin-9 Antibody

A03415 100ul
EUR 397
Description: Rabbit Polyclonal Antibody for Galectin-9 Antibody (LGALS9) detection.tested for WB in Human, Mouse, Rat.

anti- Galectin 2 antibody

FNab03310 100µg
EUR 585
  • Immunogen: lectin, galactoside-binding, soluble, 2
  • Uniprot ID: P05162
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 2

anti- Galectin 4 antibody

FNab03311 100µg
EUR 505.25
  • Recommended dilution: WB: 1:500-1:1000
  • IHC: 1:20-1:200
  • IF: 1:10-1:100
  • Immunogen: lectin, galactoside-binding, soluble, 4
  • Uniprot ID: P56470
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 4

anti- Galectin 8 antibody

FNab03312 100µg
EUR 505.25
  • Immunogen: lectin, galactoside-binding, soluble, 8
  • Uniprot ID: O00214
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 8

anti- Galectin 9 antibody

FNab03313 100µg
EUR 505.25
  • Immunogen: lectin, galactoside-binding, soluble, 9
  • Uniprot ID: O00182
  • Research Area: Immunology, Signal Transduction, Cell Division and Proliferation
Description: Antibody raised against Galectin 9

anti- Galectin-3 antibody

FNab03316 100µg
EUR 505.25
  • Recommended dilution: WB: 1:500-1:5000
  • IP: 1:200-1:2000
  • IHC: 1:20-1:200
  • IF: 1:10-1:100
  • Immunogen: lectin, galactoside-binding, soluble, 3
  • Uniprot ID: P17931
  • Research Area: Cardiovascular, Cancer, Immunology, Neuroscience, Cell Division and Prolife
  • Show more
Description: Antibody raised against Galectin-3

anti- Galectin-3 antibody

FNab03317 100µg
EUR 548.75
  • Recommended dilution: WB: 1:1000-1:5000
  • IHC: 1:50-1:200
  • IF: 1:20-1:200
  • Immunogen: lectin, galactoside-binding, soluble, 3
  • Uniprot ID: P17931
  • Research Area: Cardiovascular, Cancer, Immunology, Neuroscience, Cell Division and Proliferation
Description: Antibody raised against Galectin-3

anti- Galectin-7 antibody

FNab03318 100µg
EUR 505.25
  • Recommended dilution: WB: 1:1000-1:4000
  • IHC: 1:20-1:200
  • Immunogen: lectin, galactoside-binding, soluble, 7B
  • Uniprot ID: P47929
  • Gene ID: 653499
  • Research Area: Immunology, Cancer, Immunology, Neuroscience, Cell Division and Proliferation
Description: Antibody raised against Galectin-7

anti-Galectin-3 (6G2)

LF-MA20336 100 ug
EUR 354
Description: Mouse monoclonal to Galectin-3

Anti-Galectin 2 antibody

PAab03310 100 ug
EUR 412

Anti-Galectin 4 antibody

PAab03311 100 ug
EUR 355

Anti-Galectin 8 antibody

PAab03312 100 ug
EUR 355

Anti-Galectin 9 antibody

PAab03313 100 ug
EUR 355

Anti-Galectin-3 antibody

PAab03316 100 ug
EUR 355

Anti-Galectin-7 antibody

PAab03318 100 ug
EUR 355

Anti-Galectin-3 antibody

STJ16100369 1 mL
EUR 1047

Anti-Galectin-3 antibody

STJ16100782 100 µg
EUR 720

Anti-Galectin-2 antibody

STJ93202 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-2.

Anti-Galectin-4 antibody

STJ93203 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-4.

Anti-Galectin-7 antibody

STJ93204 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-7.

Anti-Galectin-8 antibody

STJ93205 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-8.

Anti-Galectin-9 antibody

STJ93206 200 µl
EUR 197
Description: Rabbit polyclonal to Galectin-9.

Anti-Galectin 3 antibody

STJ73098 100 µg
EUR 359

Anti-Galectin-3 antibody

STJ180106 0.1 ml
EUR 212

Anti-Galectin 3 antibody

STJ190057 200 µl
EUR 197
Description: Unconjugated Mouse monoclonal to Galectin 3 (AS1A24)

Anti-Galectin-3 antibody

STJ96945 200 µl
EUR 197
Description: Galectin-3 is a protein encoded by the LGALS3 gene which is approximately 26,1 kDa. Galectin-3 is localised to the cytoplasm and nucleus. It is involved in NF-kappaB signalling, advanced glycosylation end product receptor signalling and cell adhesion. It is a galactose-specific lectin which binds IgE and may mediate the stimulation by CSPG4 of endothelial cells migration along with the alpha-3, beta-1 integrin. It is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. Galectin-3 is expressed mainly in the colonic epithelium and is also abundantly expressed in activated macrophages. Mutations in the LGALS3 gene may result in follicular adenoma and thyroid cancer. STJ96945 was developed from clone 6G2 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous galectin-3 proteins.

Anti-Galectin-3 antibody

STJ97549 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (6B8).

Anti-Galectin-3 antibody

STJ97550 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (8D7).

Anti-Galectin-3 antibody

STJ97551 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (5D9).

Anti-Galectin-3 antibody

STJ97601 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (2F9).

Anti-Galectin-3 antibody

STJ97604 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (16E6).

Anti-Galectin-3 antibody

STJ97605 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3 (1H4).

Anti-Galectin-3 antibody

STJ97715 200 µl
EUR 197
Description: Mouse monoclonal to Galectin-3.

Anti-Galectin 8 (3E5)

YF-MA13986 100 ug
EUR 363
Description: Mouse monoclonal to Galectin 8

Anti-galectin-1 (mouse) (aa100-112) antibody

STJ72520 100 µg
EUR 359

Anti Human Galectin-3 Polyclonal Antibody

KH040
EUR 649
Description: The Anti Human Galectin-3 Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur.

Anti-Galectin-1 / Human Placental Lactogen (hPL) Monoclonal Antibody

M00470 100ug/vial
EUR 397
Description: Mouse Monoclonal Galectin-1 / Human Placental Lactogen (hPL) Antibody. Validated in IHC and tested in Human.

Galectin 1 protein

30R-1385 100 ug
EUR 224
Description: Purified recombinant Human Galectin 1 protein

Galectin 1 protein

30R-2371 50 ug
EUR 353
Description: Purified recombinant Human Galectin 1 protein

Galectin 1 protein

30R-AG003 10 ug
EUR 133
Description: Purified recombinant Human Galectin 1 protein

Galectin 1 antibody

70R-11939 100 ug
EUR 403
Description: Rabbit polyclonal Galectin 1 antibody

Galectin 1 antibody

70R-13963 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody

Galectin-1 Antibody

3596-100
EUR 316

Galectin-1 Antibody

3596-30T
EUR 146

Galectin 1 Antibody

49673-100ul 100ul
EUR 333

Galectin 1 Antibody

49673-50ul 50ul
EUR 239

Galectin 1 antibody

70R-GR002 100 ug
EUR 476
Description: Affinity purified Rabbit polyclonal Galectin 1 antibody

Galectin 1 antibody

70R-49997 100 ul
EUR 244
Description: Purified Polyclonal Galectin 1 antibody

Galectin-1 Protein

20-abx261634
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

Western Blot Analysis in Ewing Sarcoma

Western blot is an experimental approach used to analysis protein expression. In Ewing sarcoma, as in numerous totally different diseases, Western blot provides particulars in regards to the extent of protein expression in quite a few cell circumstances, in contrast with totally different tissues or upon induced molecular changes.

Based on the specific pattern of protein expression of the tissue, along with on the traits of the protein of curiosity, the antibodies and protocol of Western blot may be modified consistent with utterly totally different specs. Proper right here we describe a number of of these peculiarities in physique of Ewing sarcoma topic.

Identification of novel fibronectin-binding proteins by 2D-far Western blot in atypical enteropathogenic Escherichia coli serotype O55:H7

Atypical enteropathogenic Escherichia coli (aEPEC) is a subgroup of EPEC, which is among the many principal pathogens answerable for lethal diarrhoea in kids. In distinction with typical EPEC (tEPEC), aEPEC lack an EAF (EPEC adherence concern) plasmid (pEAF), which encodes a group of virulence-associated genes. The extracellular matrix (ECM) aspect of human cells has been reported to be an important facet throughout the interaction between host and bacterial pathogens. On this evaluation, a 2D-Far Western blot approach was carried out to identifiy the bacterial proteins that will bind to fibronectin, most likely probably the most widespread constituents of ECM. A whole of 17 protein spots have been acknowledged, along with 4 outer membrane proteins (OMPs), notably, OmpC, OmpD, OmpX and LamB. In vitro analysis have been used to search out out whether or not or not these OMPs have been involved throughout the adherence course of.

By the use of indirect immunofluorescence assays, Four OMPs could very effectively be seen on the surfaces of host cells. After incubating the cells with the recombinant proteins, the adhesion cost of the O55:H7 isolate was decreased. Furthermore, the deletion of OmpX and LamB could decrease the adhesion cost of WT. Taken collectively, a high-throughput screening approach for host ECM-binding proteins based mostly totally on 2D Far-Western blot was established, and Four outer membrane proteins acknowledged by this system have been found to be involved throughout the adherence course of.

Validation of superior reference genes for qRT-PCR and Western blot analyses in marine Emiliania huxleyi – virus model system

Objectives: To hunt for a set of reference genes for reliable gene expression analysis throughout the globally important marine coccolithophore Emiliania huxleyi -virus model system.

Methods and outcomes: Fifteen housekeeping genes (CDKA, CYP15, EFG3, POLAI, RPL30, RPL13, SAMS, COX1, GPB1-2, HSP90, TUA, TUB, UBA1, CAM3 and GAPDH) have been evaluated for his or her stability as potential reference genes for qRT-PCR using ΔCt, geNorm, NormFinder, Bestkeeper and RefFinder software program program. CDKA, TUA and TUB genes have been examined as loading controls for Western blot within the similar sample panel. Furthermore, purpose genes associated to cell apoptosis, i.e., metacaspase genes, have been utilized to validate the variety of reference genes. The analysis outcomes demonstrated that putative housekeeping genes exhibited necessary variations in every mRNA and protein content material materials all through virus an an infection. After a whole analysis with all of the algorithms, CDKA and GAPDH have been helpful as most likely probably the most regular reference genes for E huxleyi virus (EhV) an an infection cures. For Western blot, necessary variation was seen for TUA and TUB, whereas CDKA was stably expressed, in line with the outcomes of qRT-PCR.

Conclusions: CDKA and GAPDH are the best choice for gene and protein expression analysis than the alternative candidate reference genes under EhV an an infection circumstances.

Significance and affect of the look at: The regular interior administration genes acknowledged on this work will help to reinforce the accuracy and reliability of gene expression analysis and obtain notion into difficult E. huxleyi-EhV interaction regulatory networks.

Western Blot Analysis in Ewing Sarcoma

Western blot is an experimental approach used to analysis protein expression. In Ewing sarcoma, as in numerous totally different diseases, Western blot provides particulars in regards to the extent of protein expression in quite a few cell circumstances, in contrast with totally different tissues or upon induced molecular changes.

Based on the specific pattern of protein expression of the tissue, along with on the traits of the protein of curiosity, the antibodies and protocol of Western blot may be modified in accordance to utterly totally different specs. Proper right here we describe a number of of those peculiarities in physique of Ewing sarcoma topic.

Identification of novel fibronectin-binding proteins by 2D-far Western blot in atypical enteropathogenic Escherichia coli serotype O55:H7

Atypical enteropathogenic Escherichia coli (aEPEC) is a subgroup of EPEC, which is among the many principal pathogens answerable for lethal diarrhoea in kids. In distinction with typical EPEC (tEPEC), aEPEC lack an EAF (EPEC adherence concern) plasmid (pEAF), which encodes a group of virulence-associated genes. The extracellular matrix (ECM) aspect of human cells has been reported to be an important facet throughout the interaction between host and bacterial pathogens.

On this evaluation, a 2D-Far Western blot approach was carried out to identifiy the bacterial proteins that will bind to fibronectin, most likely probably the most widespread constituents of ECM. A whole of 17 protein spots have been acknowledged, along with 4 outer membrane proteins (OMPs), notably, OmpC, OmpD, OmpX and LamB. In vitro analysis have been used to search out out whether or not or not these OMPs have been involved throughout the adherence course of. By the use of indirect immunofluorescence assays, Four OMPs could very effectively be seen on the surfaces of host cells. After incubating the cells with the recombinant proteins, the adhesion cost of the O55:H7 isolate was decreased.

Furthermore, the deletion of OmpX and LamB can moreover decrease the adhesion cost of WT. Taken collectively, a high-throughput screening approach for host ECM-binding proteins based mostly totally on 2D Far-Western blot was established, and Four outer membrane proteins acknowledged by this system have been found to be involved throughout the adherence course of.

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