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A quantitative western blot method utilizing TMB

• Lisa
• July 21, 2021May 25, 2021
• 0

A quantitative western blot technique using TMB: Comparability with the usual technique

Fairly a number of molecular natural experiments carried out all by way of the world require the detection or quantification of a protein of curiosity. Western blotting is among the many hottest methods used for this purpose and offers quantitative knowledge with the assistance of specialized software program program. However, its dependence on the picture that is captured, and the background and the absence of a typical protocol forestall the tactic from being completely quantitative. To beat these obstacles, we present a straightforward and reliable assay that is very similar to the frequent technique, excluding the ultimate stage of band visualization and quantification.

We recommend that small objects of the blot that embody the protein of curiosity could be decrease and dipped in a small amount of three,3′,5,5′-Tetramethylbenzidine (TMB) decision, giving a colorimetric signal with linear dependence on the quantity of the protein. The response is stopped with H₂ SO₄ , and the signal is measured in a plate reader. This modification displays extreme linearity with out additional costs and could be utilized for every purified proteins and proteins current in a lysate. The outcomes obtained with our proposed technique have been in distinction with these obtained by the usual approach and proved to be further reliable. This textual content is protected by copyright. All rights reserved.

Anti- Galectin-1 Antibody
GWB-6F6392	GenWay Biotech	0.1 mg	Ask for price

Human Galectin-1 (Gal-1) Antibody
30005-05111	AssayPro	150 ug	EUR 215

Human Galectin-1 (LGALS1)
1-CSB-EP012882HU	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Human Galectin-1 (LGALS1)
1-CSB-EP012882HUb1	Cusabio	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Galectin-1(LGALS1) expressed in E.coli

Human Galectin-3(Galectin-3)ELISA Kit
YLA0216HU-48T	Shanghai YL Biotech	48T	Ask for price

Human Galectin-3(Galectin-3)ELISA Kit
YLA0216HU-96T	Shanghai YL Biotech	96T	Ask for price

human anti-human galectin-3 mAb(2D)
E4A09H10-2D	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human galectin-3 mAb(5F)
E4A09H10-5F	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human galectin-3 mAb(A5)
E4A09H10-A5	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human galectin-3 mAb(G2)
E4A09H10-G2	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human galectin-3 mAb(H3)
E4A09H10-H3	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human galectin-3 mAb(T1)
E4A09H10-T1	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-human galectin-3 mAb(N13)
E4A09H10-N13	EnoGene	50ug	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Anti Human Galectin-3 Polyclonal Antibody
KH040	Sceti	each	EUR 778.8
Description: The Anti Human Galectin-3 Polyclonal Antibody is available in Europe and for worldwide shipping via Gentaur.

Recombinant Human Galectin-1
7-00427	CHI Scientific	10µg	Ask for price

Recombinant Human Galectin-1
7-00428	CHI Scientific	50µg	Ask for price

Recombinant Human Galectin-1
7-00429	CHI Scientific	1mg	Ask for price

Recombinant Human Galectin-1
AP60482	SAB	100ug	EUR 449

Recombinant Human Galectin-1
HEOPP-0710	Cyagen	10ug	Ask for price

RECOMBINANT HUMAN GALECTIN-1
GWB-BEA2B0	GenWay Biotech	0.05 mg	Ask for price

Recombinant Human Galectin-1
cyt-544-10g	ProSpec Tany	10µg	EUR 60

Recombinant Human Galectin-1
cyt-544-1mg	ProSpec Tany	1mg	EUR 1350

Recombinant Human Galectin-1
cyt-544-50g	ProSpec Tany	50µg	EUR 145

Human Galectin 1 Protein
20-abx066725	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 1 mg 200 ug 50 ug

Human Galectin-1 Antibody Pair Set
ABPR-ZB244	Creative Diagnostics	5 plates, 15 plates	Ask for price
Description: Quantitative determination of Human Galectin-1

Galectin-1, human recombinant
4648-10	Biovision	each	EUR 170.4

Galectin-1, human recombinant
4648-1000	Biovision	each	EUR 1977.6

Galectin-1, human recombinant
4648-50	Biovision	each	EUR 314.4

Galectin-1 Human Recombinant
rAP-2252	Angio Proteomie	Inquiry	Ask for price

Human Galectin-1 ELISA Kit
EK5323	SAB	96 tests	EUR 599

Human Galectin-1 ELISA Kit
IHULGALS1KT	Innovative research	each	EUR 702
Description: Human Galectin-1 ELISA Kit

Mouse Monoclonal anti-human Galectin-1
hAP-1228	Angio Proteomie	50ug	EUR 400

Sheep Polyclonal anti-Human Galectin-1
hAP-5905	Angio Proteomie	50ug	EUR 400

Anti-Mouse Galectin-1 Antibody
103-M223	ReliaTech	100 µg	EUR 399
Description: The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Human Galectin-1 (LEG1) Protein
abx690252-100g	Abbexa	100 µg	EUR 837.5

Human Galectin-1 (LEG1) Protein
abx690252-20g	Abbexa	20 µg	EUR 350

Human Galectin 1 (GAL1) ELISA
KT-15605	Kamiya Biomedical Company	96 tests	EUR 952

Human Galectin-1 (LGALS1) Protein
abx670211-50ug	Abbexa	50 ug	EUR 661.2

Human Galectin 1 ELISA kit
E01A4669	BlueGene	96T	EUR 700
Description: ELISA

Human Galectin 1 ELISA kit
E01G0038-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Galectin 1 ELISA kit
E01G0038-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Galectin 1 ELISA kit
E01G0038-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Galectin 1 ELISA Kit
IHUGAL1KT	Innovative research	each	EUR 667
Description: Human Galectin 1 ELISA Kit

Galectin-10， Human
E34M155H	EnoGene	5 μg	EUR 155

Galectin-12， Human
E34M156H	EnoGene	5 μg	EUR 155

Galectin-13， Human
E34M157H	EnoGene	5 μg	EUR 155

Galectin-14， Human
E34M158H	EnoGene	5 μg	EUR 155

Galectin-16， Human
E34M159H	EnoGene	5 μg	EUR 155

Galectin-2， Human
E34M149H	EnoGene	5 μg	EUR 155

Galectin-3， Human
E34M150H	EnoGene	5 μg	EUR 155

Galectin-4， Human
E34M151H	EnoGene	5 μg	EUR 155

Galectin-7， Human
E34M152H	EnoGene	5 μg	EUR 155

Galectin-8， Human
E34M153H	EnoGene	5 μg	EUR 155

Galectin-9， Human
E34M154H	EnoGene	5 μg	EUR 155

Galectin-3 Human
GWB-1FDCC0	GenWay Biotech	0.05 mg	Ask for price

anti-human Galectin-3 antibody
10-2887	Fitzgerald	250 ug	EUR 410
Description: Purified anti-human Galectin-3 antibody

Anti-Human Galectin-3 Antibody
102-P228	ReliaTech	100 µg	EUR 245.7
Description: The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Anti-Human Galectin-3 Antibody
101-M424	ReliaTech	100 µg	EUR 399
Description: The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Anti-Human Galectin-4 Antibody
101-M425	ReliaTech	100 µg	EUR 399
Description: Galectin-4 is a member of the subfamily of galectins composed of two carbohydrate recognition domains having similar peptide chains. The galectins are a family of beta-galactoside-binding proteins having a role in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic responses. LGALS4 expression is limited to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. LGALS4 binds as an endogenous ligand to glycosphingolipids having 3-O-sulfated Gal residues and bind as well to cholesterol-3-sulfate. LGALS4 takes part in cell adhesion. LGALS4 plays a role in crosslinking the lateral cell membranes of the surface-lining epithelial cells, thus supporting epithelial integrity against mechanical stress exerted by the bowel lume.

Anti-Human Galectin-8 Antibody
101-M427	ReliaTech	100 µg	EUR 399
Description: LGALS8 is a prostate-specific antigen that is solely overexpressed in malignant tumors and thus is a supplementary specific identifier of malignancies. LGALS8 is part of the galectin gene family which facilitates both cell-cell and cell matrix interactions in a method parallel to the selectin subgroup of C-type lectins.

Anti-Human Galectin-3 Antibody
101-M747	ReliaTech	100 µg	EUR 399
Description: The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Anti-Human Galectin-7 Antibody
101-M748	ReliaTech	100 µg	EUR 399
Description: Galectin-7 is a protein that in humans is encoded by the LGALS7 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and supra-basal layers). It is moderately repressed by retinoic acid. The protein was found mainly in stratified squamous epithelium. The antigen localized to basal keratinocytes, although it was also found, albeit at lower levels, in the supra-basal layers where it concentrated to areas of cell-to-cell contact. The cellular localization and it’s striking down-regulation in cultured keratinocytes imply a role in cell–cell and/or cell–matrix interactions necessary for normal growth control.

Anti-human Galectin-3 antibody
STJ15100163	St John's Laboratory	250 µg	EUR 403.2
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation

Recombinant Human Galectin-1 (LGALS1)
CSB-EP012882HU	Cusabio	1598 mg	Ask for price

Recombinant Human Galectin-1 (LGALS1)
CSB-EP012882HUb1	Cusabio	1599 mg	Ask for price

Recombinant Human Galectin-1(LGALS1)
AP70493	SAB	1mg	EUR 1978

Recombinant Human Galectin-1 (LGALS1)
Z101845	ABM	15 µg	EUR 105
Description: Galectin-1 is a member of the galectin family, and binds B-galactosidase moieties on glycoproteins or glycolipids. Galectins are primarily involved in modulation of cell-cell and cell-matrix interactions. Galectin-1 acts as a negative regulator of immunity, promoting immune suppression and lessening the inflammatory response. Galectin-1 binds CD45, CD3 and CD4, resulting in the inhibition of CD45 phosphatase dependant dephosphorylation of lyn kinase, as well as a number of other immune related receptors. Due to its function as a negative regulator of the immune response, and role inducing apoptosis in activated Th1 and Th17 cells, it is commonly found upregulated around malignant tumours. It has also been implicated as having a role in the development of immune tolerance during pregnancy, and is highly expressed at the maternal-fetal interface. As a dimer it down-regulates neutrophils by inducing exposure of phosphatidylserine, thereby marking the cell for apoptosis. It shares approximately 88% and 90% sequence similarity with mouse and rat galectin-1, respectively. Recombinant Human Galectin-1 is a 14.9kDa protein.

Recombinant Human Galectin-1 (LGALS1)
Z101847	ABM	100 µg	EUR 270
Description: Galectin-1 is a member of the galectin family, and binds B-galactosidase moieties on glycoproteins or glycolipids. Galectins are primarily involved in modulation of cell-cell and cell-matrix interactions. Galectin-1 acts as a negative regulator of immunity, promoting immune suppression and lessening the inflammatory response. Galectin-1 binds CD45, CD3 and CD4, resulting in the inhibition of CD45 phosphatase dependant dephosphorylation of lyn kinase, as well as a number of other immune related receptors. Due to its function as a negative regulator of the immune response, and role inducing apoptosis in activated Th1 and Th17 cells, it is commonly found upregulated around malignant tumours. It has also been implicated as having a role in the development of immune tolerance during pregnancy, and is highly expressed at the maternal-fetal interface. As a dimer it down-regulates neutrophils by inducing exposure of phosphatidylserine, thereby marking the cell for apoptosis. It shares approximately 88% and 90% sequence similarity with mouse and rat galectin-1, respectively. Recombinant Human Galectin-1 is a 14.9kDa protein.

Recombinant Human Galectin-1 (LGALS1)
Z101849	ABM	1.0 mg	EUR 1135
Description: Galectin-1 is a member of the galectin family, and binds B-galactosidase moieties on glycoproteins or glycolipids. Galectins are primarily involved in modulation of cell-cell and cell-matrix interactions. Galectin-1 acts as a negative regulator of immunity, promoting immune suppression and lessening the inflammatory response. Galectin-1 binds CD45, CD3 and CD4, resulting in the inhibition of CD45 phosphatase dependant dephosphorylation of lyn kinase, as well as a number of other immune related receptors. Due to its function as a negative regulator of the immune response, and role inducing apoptosis in activated Th1 and Th17 cells, it is commonly found upregulated around malignant tumours. It has also been implicated as having a role in the development of immune tolerance during pregnancy, and is highly expressed at the maternal-fetal interface. As a dimer it down-regulates neutrophils by inducing exposure of phosphatidylserine, thereby marking the cell for apoptosis. It shares approximately 88% and 90% sequence similarity with mouse and rat galectin-1, respectively. Recombinant Human Galectin-1 is a 14.9kDa protein.

Human Galectin-1 ELISA ELISA Kit
E16HE0311	EnoGene	96T	EUR 1000

Human Galectin-1 Recombinant Protein
100-175	ReliaTech	50 µg	EUR 196.35
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.

Human Galectin-1 Recombinant Protein
100-175S	ReliaTech	10 µg	EUR 92.4
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.

Human Galectin-1 Recombinant Protein
400-015	ReliaTech	50 µg	EUR 126
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.

Recombinant Human Galectin-1 Protein
E40KMP1041	EnoGene	20ug	EUR 495

Recombinant Human Galectin-1 Protein
PROTP09382-1	BosterBio	50ug	EUR 380.4
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.

Human Galectin 10 Antibody
32652-05111	AssayPro	150 ug	EUR 215

Human Galectin 8 Antibody
33159-05111	AssayPro	150 ug	EUR 215

Human Galectin 2 Antibody
33165-05111	AssayPro	150 ug	EUR 215

Human Galectin 13 Antibody
33175-05111	AssayPro	150 ug	EUR 215

Human Galectin 1 (LGALS1) Protein
abx066725-100g	Abbexa	100 µg	EUR 512.5

Human Galectin 1 (LGALS1) Protein
abx066725-10g	Abbexa	10 µg	EUR 212.5

Human Galectin 1 (LGALS1) Protein
abx066725-50g	Abbexa	50 µg	EUR 362.5

Human Galectin 1 (LGALS1) Protein
abx670211-100g	Abbexa	100 µg	EUR 450

Human Galectin-1(LGALS1) ELISA kit
E01A4871	BlueGene	96T	EUR 700
Description: ELISA

Human Galectin-1(LGALS1) ELISA kit
E01G0448-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Galectin-1(LGALS1) ELISA kit
E01G0448-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Galectin-1(LGALS1) ELISA kit
E01G0448-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Galectin-1(LGALS1) ELISA kit
EKC33776-48T	Biomatik Corporation	48T	EUR 535.99

Human Galectin-1(LGALS1) ELISA kit
EKC33776-5x96T	Biomatik Corporation	5x96T	EUR 3637.08

Human Galectin-1(LGALS1) ELISA kit
EKC33776-96T	Biomatik Corporation	96T	EUR 765.7

Western Blot Analysis in Ewing Sarcoma

Western blot is an experimental approach used to analysis protein expression. In Ewing sarcoma, as in numerous totally different diseases, Western blot provides particulars in regards to the extent of protein expression in quite a few cell circumstances, in contrast with totally different tissues or upon induced molecular changes.

Based on the specific pattern of protein expression of the tissue, along with on the traits of the protein of curiosity, the antibodies and protocol of Western blot may be modified consistent with utterly totally different specs. Proper right here we describe a number of of these peculiarities in physique of Ewing sarcoma topic.

Identification of novel fibronectin-binding proteins by 2D-far Western blot in atypical enteropathogenic Escherichia coli serotype O55:H7

Atypical enteropathogenic Escherichia coli (aEPEC) is a subgroup of EPEC, which is among the many principal pathogens answerable for lethal diarrhoea in kids. In distinction with typical EPEC (tEPEC), aEPEC lack an EAF (EPEC adherence concern) plasmid (pEAF), which encodes a group of virulence-associated genes. The extracellular matrix (ECM) aspect of human cells has been reported to be an important facet throughout the interaction between host and bacterial pathogens. On this evaluation, a 2D-Far Western blot approach was carried out to identifiy the bacterial proteins that will bind to fibronectin, most likely probably the most widespread constituents of ECM. A whole of 17 protein spots have been acknowledged, along with 4 outer membrane proteins (OMPs), notably, OmpC, OmpD, OmpX and LamB. In vitro analysis have been used to search out out whether or not or not these OMPs have been involved throughout the adherence course of.

By the use of indirect immunofluorescence assays, Four OMPs could very effectively be seen on the surfaces of host cells. After incubating the cells with the recombinant proteins, the adhesion cost of the O55:H7 isolate was decreased. Furthermore, the deletion of OmpX and LamB could decrease the adhesion cost of WT. Taken collectively, a high-throughput screening approach for host ECM-binding proteins based mostly totally on 2D Far-Western blot was established, and Four outer membrane proteins acknowledged by this system have been found to be involved throughout the adherence course of.

Validation of superior reference genes for qRT-PCR and Western blot analyses in marine Emiliania huxleyi – virus model system

Objectives: To hunt for a set of reference genes for reliable gene expression analysis throughout the globally important marine coccolithophore Emiliania huxleyi -virus model system.

Methods and outcomes: Fifteen housekeeping genes (CDKA, CYP15, EFG3, POLAI, RPL30, RPL13, SAMS, COX1, GPB1-2, HSP90, TUA, TUB, UBA1, CAM3 and GAPDH) have been evaluated for his or her stability as potential reference genes for qRT-PCR using ΔCt, geNorm, NormFinder, Bestkeeper and RefFinder software program program. CDKA, TUA and TUB genes have been examined as loading controls for Western blot within the similar sample panel. Furthermore, purpose genes associated to cell apoptosis, i.e., metacaspase genes, have been utilized to validate the variety of reference genes. The analysis outcomes demonstrated that putative housekeeping genes exhibited necessary variations in every mRNA and protein content material materials all through virus an an infection. After a whole analysis with all of the algorithms, CDKA and GAPDH have been helpful as most likely probably the most regular reference genes for E huxleyi virus (EhV) an an infection cures. For Western blot, necessary variation was seen for TUA and TUB, whereas CDKA was stably expressed, in line with the outcomes of qRT-PCR.

Conclusions: CDKA and GAPDH are the best choice for gene and protein expression analysis than the alternative candidate reference genes under EhV an an infection circumstances.

Significance and affect of the look at: The regular interior administration genes acknowledged on this work will help to reinforce the accuracy and reliability of gene expression analysis and obtain notion into difficult E. huxleyi-EhV interaction regulatory networks.

Western Blot Analysis in Ewing Sarcoma

Western blot is an experimental approach used to analysis protein expression. In Ewing sarcoma, as in numerous totally different diseases, Western blot provides particulars in regards to the extent of protein expression in quite a few cell circumstances, in contrast with totally different tissues or upon induced molecular changes.

Based on the specific pattern of protein expression of the tissue, along with on the traits of the protein of curiosity, the antibodies and protocol of Western blot may be modified in accordance to utterly totally different specs. Proper right here we describe a number of of those peculiarities in physique of Ewing sarcoma topic.

Identification of novel fibronectin-binding proteins by 2D-far Western blot in atypical enteropathogenic Escherichia coli serotype O55:H7

Atypical enteropathogenic Escherichia coli (aEPEC) is a subgroup of EPEC, which is among the many principal pathogens answerable for lethal diarrhoea in kids. In distinction with typical EPEC (tEPEC), aEPEC lack an EAF (EPEC adherence concern) plasmid (pEAF), which encodes a group of virulence-associated genes. The extracellular matrix (ECM) aspect of human cells has been reported to be an important facet throughout the interaction between host and bacterial pathogens.

On this evaluation, a 2D-Far Western blot approach was carried out to identifiy the bacterial proteins that will bind to fibronectin, most likely probably the most widespread constituents of ECM. A whole of 17 protein spots have been acknowledged, along with 4 outer membrane proteins (OMPs), notably, OmpC, OmpD, OmpX and LamB. In vitro analysis have been used to search out out whether or not or not these OMPs have been involved throughout the adherence course of. By the use of indirect immunofluorescence assays, Four OMPs could very effectively be seen on the surfaces of host cells. After incubating the cells with the recombinant proteins, the adhesion cost of the O55:H7 isolate was decreased.

Furthermore, the deletion of OmpX and LamB can moreover decrease the adhesion cost of WT. Taken collectively, a high-throughput screening approach for host ECM-binding proteins based mostly totally on 2D Far-Western blot was established, and Four outer membrane proteins acknowledged by this system have been found to be involved throughout the adherence course of.