Designing of Western Blot Technique for Glanders
Designing of Western Blot Method for Glanders Diagnosing in Iran
Burkholderia mallei is the etiologic agent of glanders. It’s tough to diagnose this zoonotic illness in its early levels. Some strategies such because the complement fixation check (CFT) trigger some issues for veterinary authorities and monetary losses to animal homeowners as a result of false-positive outcomes. The mallein check requires applicable laboratory gear and expert personnel. To shortly and precisely diagnose the illness, particularly in areas the place animals can’t be stored, new strategies (such because the Western blot check [WBT]) needs to be used to determine the illness. This examine designed and optimized the Western blot (immunoblot) check utilizing sera from 84 glanderous equids, and the sensitivity and specificity of ELISA and CFT had been in contrast with the WBT. ELISA checks are based mostly on B. mallei antigens whereas a purified lipopolysaccharide-containing B. mallei antigen is used within the WBT.
The sensitivity and specificity of the checks had been estimated utilizing the cut-off values really useful by the check builders. The WBT and ELISA had been considerably extra particular than the CFT. The ELISA based mostly on B. mallei antigens was considerably much less delicate than the CFT. Given their comparable sensitivities and specificities, the CFT (95.7%, 98.5%), the WBT (95%, 100%) and the ELISA (85%, 100%) needs to be additional developed. The CFT continues to be the prescribed approach for serological investigation of equids for commerce functions to certify particular person animals with out glanders. Due to this fact, extra efforts needs to be made to additional enhance and optimize the WBT and ELISA checks.
 Anti-GLI-1 Antibody |
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| A07972-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for GLI-1 Antibody (ACSS1) detection. Tested with WB in Human, Mouse, Rat. |
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 Anti-Cerebellin 1 Antibody |
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| A09176-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Cerebellin 1 Antibody (CBLN1) detection. Tested with WB in Human, Mouse, Rat. |
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 Anti-DOC-1 Antibody |
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| A09467-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for DOC-1 Antibody (CDK2AP1) detection. Tested with WB in Human, Mouse. |
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 Anti-NPDC-1 Antibody |
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| A12846-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for NPDC-1 Antibody (NPDC1) detection. Tested with WB in Human, Mouse, Rat. |
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 Anti-CNG-1 Antibody |
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| A05494-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for CNG-1 Antibody (CNGA1) detection. Tested with WB in Human, Mouse, Rat. |
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 Anti-Flk-1 Antibody |
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| A00901-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Flk-1 Antibody (KDR) detection.tested for WB in Human, Mouse. |
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 Anti-EDG-1 Antibody |
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| A01502-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for EDG-1 Antibody (S1PR1) detection.tested for WB in Human, Mouse, Rat. |
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 Anti-ROBO-1 Antibody |
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| A01530-1 | BosterBio | 100ug | EUR 455 |
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Description: Rabbit Polyclonal ROBO-1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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 Anti-Bag-1 Antibody |
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| A02423-1 | BosterBio | 50 ul | EUR 397 |
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Description: Rabbit Polyclonal Bag-1 Antibody. Validated in IP, IHC and tested in Bovine, Canine, Human, Mouse, Rat. |
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 Anti-TUB 1 Antibody |
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| A02917-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-Dok-1 Antibody |
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| A03039-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Dok-1 Antibody (DOK1) detection.tested for WB in Human, Mouse, Rat. |
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 Anti-TFIIIB90-1 Antibody |
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| A03761-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for TFIIIB90-1 Antibody (BRF1) detection.tested for WB in Human, Mouse. |
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 Anti-Atrophin-1 Antibody |
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| A03828-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Atrophin-1 Antibody (ATN1) detection. Tested with WB in Human, Mouse, Rat. |
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 Anti-PARP-1 Antibody |
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| A00122-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal PARP-1 Antibody. Validated in ELISA, IP, IF, WB and tested in Human, Mouse. |
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 Anti-Presenilin 1 Antibody |
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| A00138-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Presenilin 1 Antibody (PSEN1) detection. Tested with WB, IHC in Human, Mouse, Rat. |
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 Anti-PAI-1 Antibody |
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| A00637-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for PAI-1 Antibody (SERPINE1) detection.tested for WB in Human, Mouse, Rat. |
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 Anti-ALK-1 antibody |
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| STJ91563 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Rabbit polyclonal to ALK-1. |
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Anti-ALK-1 antibody |
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| STJ96547 | St John's Laboratory | 200 µl | EUR 197 |
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Description: Rabbit polyclonal to ALK-1. |
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 Monoclonal Antibody (2E12)) Anti-Apaf-1 (human) Monoclonal Antibody (2E12) |
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| M00889-1 | BosterBio | 100ug | EUR 432 |
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Description: Rat Monoclonal Apaf-1 (human) Antibody (2E12). Validated in ELISA, IP, IF, WB and tested in Human. |
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) Anti-HO-1 Monoclonal Antibody (HO-1-2) |
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| M00253-1 | BosterBio | 1mg | EUR 397 |
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Description: Mouse Monoclonal HO-1 Antibody (HO-1-2). Validated in Flow Cytometry, IHC, WB and tested in Human, Mouse, Rat. |
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 Anti-Pyrophosphatase 1/PPA1 Antibody |
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| A07485-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-TCP-1 eta Antibody |
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| A08169-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for TCP-1 eta Antibody (CCT7) detection. Tested with WB in Human, Mouse, Rat. |
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 Anti-Relaxin 1/RLN1 Antibody |
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| A08367-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-TCP-1 zeta Antibody |
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| A09373-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for TCP-1 zeta Antibody (CCT6A) detection.tested for WB in Human, Mouse, Rat. |
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 Anti-CHST8/Galnac4St 1 Antibody |
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| A10989-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal CHST8/Galnac4St 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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 Anti-Musashi 1/Msi1 Antibody |
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| A05052-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-LOX-1/OLR1 Antibody |
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| A00760-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-Syndecan-1/SDC1 Antibody |
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| A00991-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-IRAK-1/IRAK1 Antibody |
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| A01021-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Neurexin 1/NRXN1 Antibody |
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| A01490-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Hexokinase 1/HK1 Antibody |
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| A01504-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Synaptotagmin 1/SYT1 Antibody |
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| A02314-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Syntenin-1 Monoclonal Antibody |
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| A02475-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal Antibody for Syntenin-1 Antibody (SDCBP) detection. Tested with WB in Human, Rat, Dog, Pig. |
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 Anti-Dsg1/Desmoglein 1 Antibody |
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| A02655-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Dsg1 Antibody (DSG1) detection.tested for IHC, WB in Human, Mouse, Rat. |
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 Anti-CAF-1 p150 Antibody |
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| A02732-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for CAF-1 p150 Antibody (CHAF1A) detection. Tested with WB in Human, Mouse. |
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 Anti-Talin 1/TLN1 Antibody |
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| A02859-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Islet 1/ISL1 Antibody |
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| A02969-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-TNF Receptor 1 Antibody |
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| A00294-1 | BosterBio | 200ug | EUR 522 |
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Description: Rabbit Polyclonal TNF Receptor 1 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat. |
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 Anti-Galectin 1/Lgals1 Antibody |
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| A00470-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Angiopoietin 1/ANGPT1 Antibody |
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| PA1333-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Presenilin 1 Monoclonal Antibody |
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| M00138-1 | BosterBio | 100ug | EUR 397 |
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Description: Rabbit Monoclonal Presenilin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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 Anti-Galectin 1 Monoclonal Antibody |
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| M00470-1 | BosterBio | 100ug | EUR 397 |
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Description: Rabbit Monoclonal Galectin 1 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
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 Anti-DJ-1 Monoclonal Antibody |
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| M00757-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal DJ-1 Antibody. Validated in IHC, WB and tested in Bovine, Human. |
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 Anti-Enolase 1 Monoclonal Antibody |
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| M01250-1 | BosterBio | 100ul | EUR 397 |
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Description: Anti-Enolase 1 Monoclonal Antibody tested in WB, IF, ICC, IHC, reactive to Human, rat, mouse, cow, pig, horse |
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 Anti-Dynamin 1 Monoclonal Antibody |
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| M02536-1 | BosterBio | 100ug | EUR 397 |
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Description: Rabbit Monoclonal Dynamin 1 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
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 Anti-Esrp-1 Monoclonal Antibody |
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| M06068-1 | BosterBio | 100uL | EUR 443 |
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Description: Mouse Monoclonal Esrp-1 Antibody. Validated in WB and tested in Human. |
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 Anti-Galectin 1/LGALS1 Antibody |
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| PB9240-1 | BosterBio | 100ug/vial | EUR 334 |
 Monoclonal Antibody (JSB-1)) Anti-P-glycoprotein (human) Monoclonal Antibody (JSB-1) |
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| M00049-1 | BosterBio | 125ug | EUR 586 |
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Description: Mouse Monoclonal P-glycoprotein (human) Antibody (JSB-1). Validated in IF and tested in Human. |
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 AXMIR-1 RNA oligo anti-miRNA-1-3p with Xmotif |
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| AXMIR-1 | SBI | 10 reactions | EUR 438 |
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 Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 |
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| M04431-1 | BosterBio | 100uL | EUR 385 |
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Description: Anti-BOB-1/OBF-1 Rabbit Monoclonal Antibody, Clone#RM378 tested in WB, IHC, reactive to Human |
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 Anti-CELSR3/Flamingo Homolog 1 Antibody |
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| A07204-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal CELSR3/Flamingo Homolog 1 Antibody. Validated in IF and tested in Human, Mouse, Rat. |
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 Anti-LPCAT2/Acyltransferase Like 1 Antibody |
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| A07471-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal LPCAT2/Acyltransferase Like 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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 Anti-Hyaluronan synthase 1/HAS1 Antibody |
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| A04784-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Liver Carboxylesterase 1/CES1 Antibody |
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| A01741-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-EBP50/NHERF-1/SLC9A3R1 Antibody |
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| A02427-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-IL1R2/Il 1 Rii Antibody |
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| A03106-1 | BosterBio | 1 ml | EUR 397 |
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Description: Rabbit Polyclonal IL1R2/Il 1 Rii Antibody. Validated in IP, WB and tested in Human. |
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 Anti-HIF-1 alpha/HIF1A Antibody |
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| A00013-1 | BosterBio | 100ug/vial | EUR 294 |
 Antibody) Anti-Cleaved-Notch 1 (V1754) Antibody |
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| A00033-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Cleaved-Notch 1 (V1754) Antibody (NOTCH1) detection.tested for WB in Human, Mouse, Rat. |
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 Antibody) Anti-HDAC-1 (C-terminus) Antibody |
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| A00256-1 | BosterBio | 100uL | EUR 443 |
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Description: Rabbit Polyclonal HDAC-1 (C-terminus) Antibody. Validated in IF, IHC, WB and tested in Human. |
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 Anti-VEGF Receptor 1/FLT1 Antibody |
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| A00534-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-Integrin alpha 1/ITGA1 Antibody |
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| PA1045-1 | BosterBio | 100ug/vial | EUR 334 |
/CASP1 Antibody) Anti-Caspase-1(P20)/CASP1 Antibody |
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| PA1440-1 | BosterBio | 100ug/vial | EUR 294 |
Anti-VEGF Receptor 1/FLT1 Antibody |
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| PA1966-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-Sumo 1 Rabbit Monoclonal Antibody |
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| M00631-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Sumo 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat. |
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 Anti-Cytokeratin 1 Rabbit Monoclonal Antibody |
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| M01639-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Cytokeratin 1 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
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) Anti-Mitofusin 1 Antibody (monoclonal, 3H3) |
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| M02172-1 | BosterBio | 100ug/vial | EUR 294 |
 Antibody) Anti-Phospho-Beclin-1 (Ser295) Antibody |
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| P00327-1 | BosterBio | 100ul | EUR 398 |
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Description: Rabbit Polyclonal Phospho-Beclin-1 (Ser295) Antibody. Validated in WB and tested in Human. |
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 Antibody) Anti-Phospho-Raf-1 (Ser642) Antibody |
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| P00446-1 | BosterBio | 100ul | EUR 398 |
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Description: Rabbit Polyclonal Phospho-Raf-1 (Ser642) Antibody. Validated in WB and tested in Human, Rat. |
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 Monoclonal Antibody (MEM-G/1)) Anti-HLA-G (human) Monoclonal Antibody (MEM-G/1) |
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| M01235-1 | BosterBio | 100ug | EUR 397 |
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Description: Mouse Monoclonal HLA-G (human) Antibody (MEM-G/1). Validated in IHC, WB and tested in Human. |
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 Anti-TTF-1 |
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| DB-089-1 | DB Biotech | 1 ml | EUR 750 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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, amide, human) Endothelin-1 (1-15), amide, human |
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| A1111-1 | ApexBio | 1 mg | EUR 722 |
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Description: Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium and have a key role in vascular homeostasis. |
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 Human Plasma) Haptoglobin, (Phenotype 1-1) Human Plasma |
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| 7536-1 | Biovision | EUR 321 | |
 Anti-GABA B Receptor 1/GABBR1 Antibody |
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| A07297-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-Vangl1/Vang Like Protein 1 Antibody |
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| A07587-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Vangl1 Antibody (VANGL1) detection. Tested with WB in Human, Mouse. |
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 Anti-E74 like factor 1/ELF1 Antibody |
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| A03187-1 | BosterBio | 100ug/vial | EUR 294 |
 Anti-Angiotensin Converting Enzyme 1/ACE Antibody |
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| PA2196-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody |
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| M00066-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal HMGB1/Hmg 1 Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
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 Anti-p27 KIP 1 Rabbit Monoclonal Antibody |
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| M00173-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Anti-p27 KIP 1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Rat |
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) Anti-Caveolin-1/CAV1 Antibody (monoclonal, 12C7) |
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| M00179-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-MHC class 1 Rabbit Monoclonal Antibody |
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| M00194-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal MHC class 1 Antibody. Validated in IP, IF, IHC, WB and tested in Human. |
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 Anti-Integrin beta 1 Rabbit Monoclonal Antibody |
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| M00772-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal Integrin beta 1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
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 Anti-ARG1/Arginase 1 Rabbit Monoclonal Antibody |
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| M01106-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal ARG1/Arginase 1 Antibody. Validated in IP, WB and tested in Human, Mouse, Rat. |
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 Anti-MLANA/Mart 1 Rabbit Monoclonal Antibody |
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| M02033-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal MLANA/Mart 1 Antibody. Validated in IF, WB and tested in Human. |
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) Anti-TCP-1α Monoclonal Antibody (91a) |
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| M02389-1 | BosterBio | 200ug | EUR 498 |
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Description: Rat Monoclonal TCP-1α Antibody (91a). Validated in Flow Cytometry, IP, IF, WB and tested in Human, Mouse, Rat. |
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 Anti-DSG1/Desmoglein 1 Rabbit Monoclonal Antibody |
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| M02655-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal DSG1/Desmoglein 1 Antibody. Validated in IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
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 Antibody) Anti-Phospho-Glycogen Synthase 1 (S645) Antibody |
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| P03512-1 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Antibody for Phospho-Glycogen Synthase 1 (S645) Antibody (GYS1) detection.tested for WB in Human, Mouse, Rat. |
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) Anti-Musashi 1/Msi1 Antibody (monoclonal, 2B9) |
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| M05052-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-Visinin-like Protein 1 Monoclonal Antibody |
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| M06959-1 | BosterBio | 100ul | EUR 397 |
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Description: Mouse Monoclonal Visinin-like Protein 1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
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) Hemokinin 1 (human) |
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| B5318-1 | ApexBio | 1 mg | EUR 340 |
, human recombinant protein) Neuregulin/Heregulin-1? (NRG-1?/HRG-1?), human recombinant protein |
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| P1054-1 | ApexBio | 1 mg | EUR 3947 |
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Description: Neuregulin (NRG) is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells and plays an important role in heart structure and function through inducing cardiomyocyte differentiation |
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 miRZip-1 anti-miR-1 microRNA construct |
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| MZIP1-PA-1 | SBI | Bacterial Streak | EUR 684 |
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 Anti-EBV/LMP-1 |
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| DB-060-1 | DB Biotech | 1 ml | EUR 750 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-EBV/LMP-1 |
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| DB-061-1 | DB Biotech | 1 ml | EUR 750 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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 Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody |
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| A01268-1 | BosterBio | 100ug/vial | EUR 334 |
 Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody |
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| M00522-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal AFP/Alpha 1 Fetoprotein Antibody. Validated in IP, IF, WB and tested in Human. |
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 Anti-SERPINA1/Alpha 1 Antitrypsin Rabbit Monoclonal Antibody |
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| M00720-1 | BosterBio | 100ug/vial | EUR 397 |
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Description: Rabbit Monoclonal SERPINA1/Alpha 1 Antitrypsin Antibody. Validated in IP, IF, WB and tested in Human. |
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Evaluating Host Responses to Helicobacter pylori Utilizing ELISA and Western Blot
Western blot and enzyme-linked immunosorbent assay (ELISA) are antibody-mediated methods that are extensively used for the detection and characterization of alterations in host protein expression following H. pylori an infection . Each methods are extremely particular and delicate for protein detection, with Western blot detection sensitivity as little as picogram quantities of the protein of curiosity, whereas the standard ELISA detection vary is 0.01-0.1 ng. Right here we offer an experimental instance to display the appliance of those methods for the dedication of macrophage inflammatory responses following H. pylori an infection .
Western Blot as a Help Method for Immunohistochemistry to Detect Programmed Cell Loss of life Ligand 1 Expression
Antibody choice and optimization are essential to ensure correct and reproducible outcomes when utilizing such antibodies for functions resembling western blot evaluation and immunohistochemistry (IHC). That is particularly vital when deciding on good candidate antibodies that will probably be used for most cancers immunotherapy diagnostics and analysis. On this chapter, we describe a Western Blot approach as help methodology for the choice and validation of Programmed Cell Loss of life Ligand 1 (PD-L1) antibodies that may be subsequently utilized in immunohistochemistry functions.
Western Blot is a delicate, particular, and extensively out there protein characterization approach, used for the detection of particular antigens. PD-L1 is a significant immune checkpoint protein that mediates antitumor immune suppression and response, which is routinely detected utilizing IHC in formalin-fixed and paraffin-embedded tissues as a part of most cancers scientific diagnostic workflows. For that reason, it’s vital to outline and choose the greatest antibody clones and validate them utilizing totally different methods as a way to have a dependable detection of optimistic staining when these antibodies are utilized in IHC.
Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
Cardiac, neuronal and renal tubular epithelial cells are the most metabolically lively cells within the physique. Their destiny relies upon largely on their mitochondria as the first power producing system which participates within the management of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a trademark of many continual ailments together with diabetic nephropathy. A drop in mitochondrial bioenergetics effectivity is commonly related to altered expression of respiratory chain complexes.
Furthermore, current research display that mobile proteins can shuttle to mitochondria and modify their operate straight. Right here we illustrate two mitochondria isolation protocols; one is really useful if the purity of the mitochondrial fraction is a precedence resembling if the mitochondrial localization of a protein needs to be validated, the opposite if a excessive yield of intact practical mitochondria is required for practical research and quantitative Western blotting. Subsequent, we offer an in depth protocol for Western blotting of remoted mitochondria and renal cortex both to show the purity of remoted fractions or to quantify complexes of the mitochondrial respiratory chain. We used this strategy to determine classically cell membrane sure angiotensin II receptors in mitochondria and to check the impact of those receptors on mitochondrial operate in early levels of diabetic nephropathy.
Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis grownup worms by Western blot
Earlier research confirmed that crude antigens from Trichinella spiralis grownup worms (AW) will be acknowledged by mouse an infection sera at Eight days submit an infection. The goal of this examine was to determine the early diagnostic antigenic bands in soluble proteins from T. spiralis AW by Western blot utilizing early an infection sera. The affecting components of grownup restoration had been firstly noticed on this examine, and the outcomes confirmed that the utmost variety of adults was collected from small gut when the feminine BALB/c mice had been orally contaminated with 4000 ML and sacrificed at three days submit an infection.
The outcomes of Western blot evaluation confirmed that seven protein bands (31, 35.1, 39, 40.6, 41.9, 47 and 50.6 kDa) could possibly be acknowledged by early an infection sera as early as at 8-10 days submit an infection, and had been strongly reacted with mouse an infection sera at 11-12 days submit an infection. Our outcomes recommended that the seven protein bands of T. spiralis AW soluble proteins is likely to be the early expressed antigens in the course of the intestinal stage of Trichinella an infection and due to this fact have potential worth for the early analysis of trichinellosis.
 L Wnt-3A cells |
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| S0011001 | Addexbio | One Frozen vial | EUR 455 |
 Protein Wnt-3a Polyclonal Antibody |
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| 42596-100ul | SAB | 100ul | EUR 333 |
) Human Protein Wnt-3a (WNT3A) |
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| 1-CSB-EP026136HU | Cusabio |
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Description: Recombinant Human Protein Wnt-3a(WNT3A) expressed in E.coli |
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) Mouse Protein Wnt-3a (Wnt3a) |
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| 1-CSB-EP026136MO | Cusabio |
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Description: Recombinant Mouse Protein Wnt-3a(Wnt3a) expressed in E.coli |
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Human Protein Wnt-3a (WNT3A) |
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| 1-CSB-RP155494h | Cusabio |
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Description: Recombinant Human Protein Wnt-3a(WNT3A),partial expressed in E.coli |
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 Antibody) Protein Wnt-3a (WNT3A) Antibody |
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| abx218011-100ug | Abbexa | 100 ug | EUR 439 |
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Protein Wnt-3a (WNT3A) Antibody |
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| abx224220-100ug | Abbexa | 100 ug | EUR 411 |
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) Protein Wnt-3a Antibody (Biotin) |
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| 20-abx105841 | Abbexa |
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) Protein Wnt-3a Antibody (FITC) |
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| 20-abx107257 | Abbexa |
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) Protein Wnt-3a Antibody (HRP) |
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| 20-abx108677 | Abbexa |
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Protein Wnt-3a (WNT3A) Antibody |
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| abx117009-100ug | Abbexa | 100 ug | EUR 467 |
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Protein Wnt-3a (WNT3A) Antibody |
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| 20-abx136135 | Abbexa |
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Protein Wnt-3a (WNT3A) Antibody |
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| 20-abx000791 | Abbexa |
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 Antibody) Wnt Family Member 3A (WNT3A) Antibody |
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| 20-abx211813 | Abbexa |
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Wnt Family Member 3A (WNT3A) Antibody |
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| 20-abx211920 | Abbexa |
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 Protein Wnt-3a Polyclonal Conjugated Antibody |
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| C42596 | SAB | 100ul | EUR 397 |
 Wnt-C59 |
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| A8685-5 | ApexBio | 5 mg | EUR 148 |
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Description: Wnt-C59 is a selective inhibitor of Wnt signaling with IC50 value of 74 pM [1]. |
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 Wnt Antagonist, C59 |
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| 2063-5 | Biovision | EUR 359 | |
 ELISA kit) Human Protein Wnt-3a(WNT3A) ELISA kit |
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| CSB-EL026136HU-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
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Description: Quantitativesandwich ELISA kit for measuring Human Protein Wnt-3a (WNT3A) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human Protein Wnt-3a(WNT3A) ELISA kit |
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| 1-CSB-EL026136HU | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human Protein Wnt-3a(WNT3A) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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 ELISA kit) Mouse Protein Wnt-3a(WNT3A) ELISA kit |
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| CSB-EL026136MO-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
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Description: Quantitativesandwich ELISA kit for measuring Mouse Protein Wnt-3a (WNT3A) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Mouse Protein Wnt-3a(WNT3A) ELISA kit |
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| 1-CSB-EL026136MO | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Mouse Protein Wnt-3a(WNT3A) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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 ELISA Kit) Mouse Protein Wnt-3a (WNT3A) ELISA Kit |
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| abx255085-96tests | Abbexa | 96 tests | EUR 746 |
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 ELISA Kit) Human Protein Wnt-3a (WNT3A) ELISA Kit |
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| abx251482-96tests | Abbexa | 96 tests | EUR 746 |
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 ELISA kit for Mouse Protein Wnt-3a |
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| EK4364 | SAB | 96 tests | EUR 553 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Protein Wnt-3a in samples from serum, plasma, tissue homogenates and other biological fluids. |
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 ELISA kit for Human Protein Wnt-3a |
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| EK4365 | SAB | 96 tests | EUR 553 |
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Description: Enzyme-linked immunosorbent assay kit for quantification of Human Protein Wnt-3a in samples from serum, plasma, tissue homogenates and other biological fluids. |
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 Mouse Wnt3a/ Protein Wnt-3a ELISA Kit |
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| E1594Mo | Sunlong | 1 Kit | EUR 571 |
 Human WNT3A/ Protein Wnt-3a ELISA Kit |
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| E2688Hu | Sunlong | 1 Kit | EUR 571 |
 ELISA Kit) Human WNT3A(Protein Wnt-3a) ELISA Kit |
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| EH2146 | FN Test | 96T | EUR 567.6 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
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 Mouse Protein Wnt- 3a, Wnt3a ELISA KIT |
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| ELI-06995m | Lifescience Market | 96 Tests | EUR 865 |
 Chicken Protein Wnt- 3a, WNT3A ELISA KIT |
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| ELI-06996c | Lifescience Market | 96 Tests | EUR 928 |
 Human Protein Wnt- 3a, WNT3A ELISA KIT |
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| ELI-06997h | Lifescience Market | 96 Tests | EUR 824 |
Human Protein Wnt-3a (WNT3A) ELISA Kit |
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| abx570841-96tests | Abbexa | 96 tests | EUR 668 |
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Mouse Protein Wnt-3a (WNT3A) ELISA Kit |
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| abx575000-96tests | Abbexa | 96 tests | EUR 668 |
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 ELISA Kit) Chicken Protein Wnt-3a (WNT3A) ELISA Kit |
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| abx519656-96tests | Abbexa | 96 tests | EUR 911 |
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 ELISA Kit) Mouse Wnt3a(Protein Wnt-3a) ELISA Kit |
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| EM0737 | FN Test | 96T | EUR 567.6 |
|
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.188 ng/ml |
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 5-HT-3A Antibody |
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| 34152-100ul | SAB | 100ul | EUR 252 |
5-HT-3A Antibody |
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| 34152-50ul | SAB | 50ul | EUR 187 |
5-HT-3A Antibody |
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| DF3502 | Affbiotech | 200ul | EUR 304 |
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Description: 5-HT-3A Antibody detects endogenous levels of total 5-HT-3A. |
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 5- HT- 3A Antibody |
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| ABD3502 | Lifescience Market | 100 ug | EUR 438 |
) ELISA kit for Mouse Protein Wnt-3a (WNT3A) |
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| KTE70008-48T | Abbkine | 48T | EUR 332 |
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Description: Quantitative sandwich ELISA for measuring Mouse Protein Wnt-3a (WNT3A) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Mouse Protein Wnt-3a (WNT3A) |
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| KTE70008-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2115 |
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Description: Quantitative sandwich ELISA for measuring Mouse Protein Wnt-3a (WNT3A) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Mouse Protein Wnt-3a (WNT3A) |
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| KTE70008-96T | Abbkine | 96T | EUR 539 |
|
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Description: Quantitative sandwich ELISA for measuring Mouse Protein Wnt-3a (WNT3A) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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) ELISA kit for Human Protein Wnt-3a (WNT3A) |
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| KTE60023-48T | Abbkine | 48T | EUR 354 |
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Description: Quantitative sandwich ELISA for measuring Human Protein Wnt-3a (WNT3A) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Protein Wnt-3a (WNT3A) |
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| KTE60023-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2252 |
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Description: Quantitative sandwich ELISA for measuring Human Protein Wnt-3a (WNT3A) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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ELISA kit for Human Protein Wnt-3a (WNT3A) |
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| KTE60023-96T | Abbkine | 96T | EUR 572 |
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Description: Quantitative sandwich ELISA for measuring Human Protein Wnt-3a (WNT3A) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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 Growth Factor Set IV, StemBoost? |
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| K426-5 | Biovision | EUR 854 | |
 Antibody) Human Growth Differentiation Factor 5 (GDF-5) Antibody |
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| 30011-05111 | AssayPro | 150 ug | EUR 261 |
 Fetal Bovine Serum,Low IgG , < 5 ug/ml |
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| F0710-050 | GenDepot | 500 ml | EUR 1198 |
 Growth/differentiation factor 5 Polyclonal Antibody |
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| 42178-100ul | SAB | 100ul | EUR 333 |
) Mouse Growth/differentiation factor 5 (Gdf5) |
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| 1-CSB-EP009349MO | Cusabio |
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Description: Recombinant Mouse Growth/differentiation factor 5(Gdf5) expressed in E.coli |
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 Antibody) Fibroblast Growth Factor 5 (FGF5) Antibody |
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| 20-abx009268 | Abbexa |
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 Antibody) Growth Differentiation Factor 5 (GDF5) Antibody |
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| abx026572-400ul | Abbexa | 400 ul | EUR 523 |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| abx026572-80l | Abbexa | 80 µl | EUR 286 |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx006710 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx176717 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx176718 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx103263 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx103264 | Abbexa |
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Fibroblast Growth Factor 5 (FGF5) Antibody |
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| 20-abx123595 | Abbexa |
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Fibroblast Growth Factor 5 (FGF5) Antibody |
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| 20-abx128010 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx128606 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx109840 | Abbexa |
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Fibroblast Growth Factor 5 (FGF5) Antibody |
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| 20-abx172410 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx172684 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx172685 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| abx031516-400ul | Abbexa | 400 ul | EUR 523 |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| abx031516-80l | Abbexa | 80 µl | EUR 286 |
|
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx320075 | Abbexa |
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Fibroblast Growth Factor 5 (FGF5) Antibody |
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| abx233094-100ug | Abbexa | 100 ug | EUR 509 |
|
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| 20-abx327566 | Abbexa |
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Fibroblast Growth Factor 5 (FGF5) Antibody |
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| 20-abx323550 | Abbexa |
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Growth Differentiation Factor 5 (GDF5) Antibody |
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| abx432738-200ul | Abbexa | 200 ul | EUR 286 |
|
|||
) Recombinant Fibroblast Growth Factor 5 (FGF5) |
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| 4-RPA357Hu01 | Cloud-Clone |
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Description: Recombinant Human Fibroblast Growth Factor 5 expressed in: E.coli |
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) Recombinant Growth Differentiation Factor 5 (GDF5) |
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| 4-RPC110Hu01 | Cloud-Clone |
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Description: Recombinant Human Growth Differentiation Factor 5 expressed in: E.coli |
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Recombinant Growth Differentiation Factor 5 (GDF5) |
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| 4-RPC110Hu02 | Cloud-Clone |
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Description: Recombinant Human Growth Differentiation Factor 5 expressed in: E.coli |
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Recombinant Growth Differentiation Factor 5 (GDF5) |
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| 4-RPC110Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Growth Differentiation Factor 5 expressed in: E.coli |
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 Antibody) 5-Hydroxytryptamine Receptor 3A (5-HT3A) Antibody |
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| 20-abx013887 | Abbexa |
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, human recombinant) Epidermal Growth Factor (EGF), human recombinant |
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| P1008-.5 | ApexBio | 500 µg | EUR 313 |
|
Description: Human epidermal growth factor (EGF) is a member of the EGF-family and plays an important role in the regulation of cell growth, proliferation, and differentiation through binding to its receptor EGFR. |
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Epidermal Growth Factor (EGF), human recombinant |
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| P1008-5 | ApexBio | 5 mg | EUR 1372 |
|
Description: Human epidermal growth factor (EGF) is a member of the EGF-family and plays an important role in the regulation of cell growth, proliferation, and differentiation through binding to its receptor EGFR. |
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 Wnt3a ELISA Kit| Mouse Protein Wnt-3a ELISA Kit |
|||
| EF013348 | Lifescience Market | 96 Tests | EUR 689 |
 Recombinant Human Protein Wnt-3a Protein, His, E.coli-100ug |
|||
| QP8838-ec-100ug | EnQuireBio | 100ug | EUR 408 |
 Recombinant Human Protein Wnt-3a Protein, His, E.coli-10ug |
|||
| QP8838-ec-10ug | EnQuireBio | 10ug | EUR 200 |
 Recombinant Human Protein Wnt-3a Protein, His, E.coli-1mg |
|||
| QP8838-ec-1mg | EnQuireBio | 1mg | EUR 1632 |
 Recombinant Human Protein Wnt-3a Protein, His, E.coli-200ug |
|||
| QP8838-ec-200ug | EnQuireBio | 200ug | EUR 634 |
 Recombinant Human Protein Wnt-3a Protein, His, E.coli-500ug |
|||
| QP8838-ec-500ug | EnQuireBio | 500ug | EUR 1060 |
 Recombinant Human Protein Wnt-3a Protein, His, E.coli-50ug |
|||
| QP8838-ec-50ug | EnQuireBio | 50ug | EUR 263 |
 5-HT-3A Blocking Peptide |
|||
| DF3502-BP | Affbiotech | 1mg | EUR 195 |
 5-HT-3A Conjugated Antibody |
|||
| C34152 | SAB | 100ul | EUR 397 |
) Epidermal Growth Factor Receptor Peptide (985-996) |
|||
| A1021-5 | ApexBio | 5 mg | EUR 189 |
|
Description: EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by the binding of its specific ligands, including epidermal growth factor and transforming growth factor ?. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. |
|||
) Insulin-like Growth Factor I (57-70) |
|||
| 5-01399 | CHI Scientific | 4 x 5mg | Ask for price |
) Insulin-Like Growth Factor II (69-84) |
|||
| 5-01400 | CHI Scientific | 4 x 1mg | Ask for price |
 Platelet-Derived Growth Factor Receptor Substrate 2 |
|||
| 5-01775 | CHI Scientific | 4 x 1mg | Ask for price |
 (mouse)) Prepro-Nerve Growth Factor (99-115) (mouse) |
|||
| 5-01801 | CHI Scientific | 4 x 1mg | Ask for price |
Epidermal Growth Factor Receptor Peptide (985-996) |
|||
| 5-01114 | CHI Scientific | 4 x 1mg | Ask for price |
