InstantBlue is a ready-to-use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action stains proteins in 15 minutes and leaves a clear background that eliminates the need to fix, wash, or destain. InstantBlue is formulated for safe use and easy removal.
InstantBlue is ready to use straight from the bottle and comes in convenient 1-litre premixed volumes. Its exclusive patented mechanism starts staining in moments and results are achieved in 15 minutes. Some traditional precast and homemade Coomassie R-250 stains can take 3 hours or more to completely stain the gels.
There is no need to wash, set, microwave, or bleach the gels when using InstantBlue. Homemade stains often require all of these steps, and most pre-made solutions require at least one. InstantBlue uses a one-step protocol and can be fully destained using standard procedures. InstantBlue’s acetic acid-free formulation does not methylate or acetylate protein during staining, making it fully compatible with mass spectrometry.
Unmatched sensitivity allows the detection of protein levels as low as 5 ng per band (BSA) when stained overnight. This is due to InstantBlue’s protein-specific staining, which provides a clear background and improves the signal-to-noise ratio of staining. Gels can be left in InstantBlue for weeks and still come out with a crystal clear background, all without destaining.
InstantBlue is non-toxic and sink-disposable due to its methanol-free composition. Other stains require solvent removal procedures and may require microwaves, which produce dangerous fumes. InstantBlue’s effective staining and safe composition mean there’s no need for microwaves, solvent removal, or fume hoods. The lack of methanol also means that there is no shrinkage of the gel. Inexpensive staining with only 25 mL per mini gel allows you to use less InstantBlue. Homemade solutions and some premixed solutions require around 50-100 ml of dye per gel. InstantBlue will stain at least 40 minigels per bottle.
Features and benefits:
- Ultra-fast staining – results in 15 minutes or less
- One-step procedure – no washing, setting, microwaving or bleaching
- High sensitivity: 5 ng bands detectable*
- Light background: high signal-to-noise ratio
- Flexible – no overstaining
- Quantitative: batch-to-batch consistency
- Efficient: only 25 ml per gel
- Safe composition – Non-toxic. No fume hood or solvent removal required
- No methanol: no gel shrinkage or protein methylation
- Acetic Acid-Free – No protein acetylation
- Compatible with MS 1 – Assignable. No residual methylation or acetylation
Storage: Store at 4C.
This product is manufactured by Abcam and distributed by Novus Biologicals. This product is for research use only and is not approved for human or clinical diagnostic use. This product is warranted for 1 year from the date of receipt and this statement voids any warranty period mentioned in the limitations section of this product data-sheet. Please contact firstname.lastname@example.org if you have any questions.
This product is for research use only and is not approved for human or clinical diagnostic use. Support products are guaranteed for 6 months from the date of receipt.