Rapid Canine Brucella Ab Test Kit
George described the Rapid Slide Agglutination Test (RSAT) for the diagnosis of Brucella Canis and Carmichael (Proc. Council Res. Workers in Animal Disease, November 1973). The basis of the test is the direct agglutination of the dead antigen stained by Brucella canis antibodies.
Canine brucellosis is a chronic Brucella canis infection that causes generalized lymphadenitis and Mild to severe reproductive symptoms. Prostatitis, epididymitis, scrotal dermatitis, testicular atrophy, and impotence can be present in men; abortion typically in the last two weeks of gestation, reabsorption, infertility, and vaginal discharge may be present in women.
Brucella Canis is infectious to humans. Caution should be exercised when handling sera to be tested.
The test has been shown to presumptively diagnose Brucella canis infection by detecting antibody that forms 1 to 4 weeks after infection. The basis for the serodiagnosis of canine brucellosis is 2-Mercaptoethanol Tube Agglutination Test (2ME-TAT) and Rapid Slide Agglutination Test (RSAT). 2ME-TAT and RSAT have shown an excellent correlation in cases of experimental infection dogs.
In in field situations, it has been recognized that an occasional healthy dog, culturally negative for Brucella canis will react positively on the RSAT, but not on the 2ME-TAT. 2-Mercaptoethanol – Fast The slide agglutination test (2ME-RSAT) has been developed in an attempt to eliminate discrepancies between RSAT and 2-ME-TAT.
Certain nonspecific agglutinins, which are reported to occur in the serum of normal dogs, are shed from dogs sera when 2-mercaptoethanol is used in the 2ME-TAT. Due to this occurrence, 2- Mercaptoethanol is used in the modified rapid slide agglutination test procedure.
The reagents are used to diagnose suspected Brucella canis infection in dogs. Positive results require additional confirmation.
The antigen and accompanying antiserum have been standardized and must be used together. Store components at 2-7C (35-45F). Do not allow the reagents to remain at room temperature for periods of time. Do not freeze.
The serum is required. The test cannot be used with whole blood or plasma.